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Rapid Immuno-Migration (RIM) Assay for detection of Parvovirus antigen in the faeces of dogs.
Point-of-Care test with results in 10 minutes or less.
Recommendations for use:
Confirmation of infection with Parvovirus in sick dogs.
1. SAMPLE COLLECTION
Remove the top portion, containing the cotton swab, from a sample extraction pipette.
Dispense Extraction Buffer into the base of the sample extraction pipette until the buffer reaches the graduation mark.
Taking the top portion of the sample extraction pipette, coat the cotton swab with a thin layer of faeces from a stool sample, or an anal swab.
Insert the swab into the dispensed extraction buffer in the base of the sample extraction pipette. Securely fit the top portion onto the base and tap to mix for 5 seconds to ensure good sample extraction.
2. SAMPLE DISPENSING
Tear open the pouch provided and place the test device on a flat horizontal surface during the migra- tion of the samples.
Snap the top of the sample extraction pipette (at the blue line) containing the extracted faecal sample.
Invert and hold the sample extraction pipette in a vertical (upright) position and squeeze the base gently to dispense five drops of sample to the sample well (Window (1)).
If migration does not pass Window (2) by one minute, add one additional drop of sample in Window (1).
3. READING TEST
Wait 10 minutes, then observe the presence or absence of purple bands in Windows (2) and (3).
Sample results are read in Window (2). The control band is read in Window (3).
The test is complete and may be read before
10 minutes if purple bands are visible in both
Windows (2) and (3).
The presence of a purple band only in Window (3) before 10 minutes does not mean that the test is com- plete. A purple band in Window (2) may develop slower
than the control purple band in Window (3).
Negative - A sample is negative for Canine Parvovirus antigen, if there is no purple band visible in Window
(2) and a purple band in Window (3).
Positive - A sample is positive for Canine Parvovirus antigen if there is one purple band visible in Window
(2) and one purple band in Window (3).
Note : A test is valid if a purple band is present in Window (3). If there is no purple band in Window (3), the test is invalid and should be repeated.
Remember : A test result should always be interpreted in the context of all available clinical information and history for the dog being tested.