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Poulvac® Laryngo A20

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  • Live attenuated infectious laryngotracheitis (ILT) virus vaccine produced in specific pathogen free eggs; egg adapted. Each dose contains infectious laryngotracheitis virus (A20 strain; ≥ 103.5 pfu). The virus strain used (clone A20) is characterised by extremely low mortality and low morbidity after tracheal inoculation of susceptible chickens.

  • Principally for the preventive vaccination of young stock against infectious laryngotracheitis

  • Correct storage and administration of the vaccine is essential. Freeze dried vials. The vaccine will remain potent until the expiry date if stored below –5°C (frozen). Protect from light. Reconstituted vaccine. Once reconstituted, the vaccine must be used within two hours. Special precautions should be taken to avoid heat and light damage to the vaccine during use, especially in open sided iron shedding in summer. If temperatures are expected to be over 25°C, small volumes of vaccine should be reconstituted and used quickly. Covered insulated containers containing ice should be used for the movement of vaccine vials from the main storage area to areas of use around the farm.

    Vaccination is recommended at 7 days of age to provide early protection against ILT infection. Broiler birds held beyond 7 weeks of age may need to be revaccinated. Flocks up to 15 weeks of age can be safely vaccinated by eyedrop or water methods during an outbreak of ILT. Start by vaccinating birds farthest from the infected area and then work towards the sick birds.

    Before reconstitution. The vaccine will remain potent until the expiry date if stored below –5°C (frozen). Reconstituted vaccine. Once reconstituted, the vaccine must be used immediately. Special precautions should be taken to avoid heat and light damage to the vaccine during use. If ambient temperatures are expected to be high, small volumes of vaccine should be reconstituted and used quickly. Covered insulated containers containing ice should be used for the movement of vaccine vials from the main storage area to areas of use around the farm. Two methods of administration are recommended: eye drop and drinking water.

    Eye drop administration. Reconstitute the vaccine with Poulvac Eyedrop Diluent, supplied in a plastic bottle which becomes the dropper bottle when the provided dropper cap is fitted. Reconstitution of 500 dose vaccine. The diluent (approximately 30 mL per bottle) is added to the virus in two vials of vaccine, and after shaking the contents of both are poured into the diluent bottle and the dropper cap fitted. Reconstitution of 750 dose vaccine. Withdraw 7.5 mL of diluent (containing approximately 30 mL per bottle) and discard. Add the remaining diluent from the bottle to the virus in one vial of vaccine. After shaking, the contents are poured into the diluent bottle and the dropper cap fitted. Reconstitution of 1,000 dose vaccine. The diluent (approximately 30 mL per bottle) is added to the virus in one vial of vaccine, and after shaking, the contents are poured into the diluent bottle and the dropper cap fitted. Reconstitution of 1,500 dose vaccine. Withdraw 7.5 mL of diluent (containing approximately 30 mL per bottle) from each of two diluent bottles (total of 15 mL) and discard. Withdraw 1 mL of the remaining diluent from each bottle (total of 2 mL). Add to the virus in one vial of vaccine and, after shaking it, draw up the total volume of reconstituted vaccine virus into a syringe. Determine the total volume from graduations on the syringe and deliver half of this amount to each of the two diluent bottles and fit a dropper cap to each. Reconstitution of 2,000 dose vaccine. Withdraw 1 mL of diluent (approximately 30 mL per bottle) from each of two diluent bottles (total of 2 mL). Add the 2 mL of diluent to the virus in the vial, and after shaking, draw up the total volume of reconstituted vaccine virus into a syringe. Determine the total volume from graduations on the syringe and deliver half of this amount to each of the two diluent bottles and fit a dropper cap to each. If ambient temperatures are expected to be high, one vial of vaccine at a time should be reconstituted and used immediately.

    Administration. Hold the bird with the eye upward and apply 1 drop (0.03 mL) to the eye. Make sure the drop of vaccine spreads over the eye before releasing the bird. It is an important part of successful technique to hold the bird in such a way that the droplet covers the eye surface and does not run off.

    Drinking water administration. Reconstitution. Whenever available, clean rainwater or tank water containing 2.5 g of instant skim milk powder/L should be used as a diluent for reconstitution. The vaccine should be prepared immediately before use by tearing the cap off the vial, removing the rubber stopper and resuspending the virus with prechilled diluent. After shaking, the reconstituted vaccine is poured into the 3 to 5 L of skim milk solution (2.5 g of the instant skim milk/L of chilled water).

    Administration.

    Early morning administration is recommended. At this time there is rapid consumption of water by the birds in conditions of temperature and light most favourable for effective and minimum stress to the flock, early morning administration also ensures that the vaccine is reconstituted in cool water. Drinking water vaccination with Poulvac Laryngo A20 can be successfully applied through water lines in sheds with trough or fixed drinkers operating on gravity feed from a holding tank. It can also be applied directly to drinkers. For a three day period prior to vaccination, the volume of water consumed by the chickens is calculated over a two hour period, preferably in the early morning. This gives a good indication of the volume of drinking water to be used during vaccination. N.B. During this three day period no disinfectants or drugs are to be used in the drinking system.

    • a. Medication tank method.
      • 1. Clean and rinse all drinking troughs thoroughly but do not use disinfectants.
      • 2. Empty header tank and fill with sufficient fresh water calculated to last chickens for two hours. Turn off the water supply.
      • 3. Add skim milk powder (2.5 g/L) and mix thoroughly. Premix the skim milk and a small volume of water before adding to the tank. This acts as a stabiliser and protects the live virus in the vaccine.
      • 4. Add the prepared vaccine to the header tank and stir thoroughly to disperse it in the skim milk and water mixture.
      • 5. Drain the drinking points until the white coloured skim milk and vaccine and water mixture appears and fills the drinkers.
      • 6. Ensure that all birds drink the vaccine supply by driving them gently towards the water troughs. The vaccine mixture should be consumed within two hours.
      • 7. Do not supply water until all the vaccine mixture has been consumed.
    • b. Direct dispensing into drinkers method.
      • 1. Clean and rinse the water trough then turn off the water supply. Do not use disinfectants.
      • 2. Calculate the number of doses required.
      • 3. Prepare the correct volume of chilled water and add skim milk at 2.5 g/L. Dissolve thoroughly. Prepare the correct number of doses as described above (Drinking water administration, Reconstitution) and mix thoroughly with the skim milk.
      • 4. Using a clean plastic watering can or bucket distribute the vaccine mixture evenly to the water troughs. Keep replenishing the troughs until the total volume in the large container is used.
      • 5. Make sure the birds drink the vaccine mixture by driving them gently towards the water troughs. Total consumption of the vaccine should be completed within two hours. The birds must not be allowed access to any other water supply until the vaccination is completed.
  • Simultaneous administration of Poulvac Laryngo A20 and Infectious Bronchitis Vaccine is not recommended. Only birds in good health should be vaccinated. The presence of either concurrent infections (e.g. mycoplasmosis, coryza) or husbandry stress factors may cause a reaction.

    The use of disinfectants or antiseptics in or on any equipment used for the preparation of Poulvac Laryngo A20 must be avoided. Sanitising chemicals in the farm water supply should not be used for three days prior to vaccination. All recommendations on the storage and care of the unreconstituted vaccine must be followed. Avoid heat and light damage to the vaccine during use. Reconstituted vaccine must be prepared, stored and transported as recommended and must be used immediately after reconstitution. Transient eye reactions in chickens may result from accidental or deliberate ocular inoculation. All birds must receive the full dose of living vaccine to be fully protected.

    Concurrent administration with infectious bronchitis vaccine. Administration to unhealthy birds, e.g. mycoplasmosis or coryza infected birds, which may have severe reactions to the vaccine

  • Research has shown that ILT vaccine viruses can recombine to potentially form more virulent strains of ILT virus. To minimise the possibility of this happening, ILT virus vaccines originating from genetically distinct ILT virus strains should not be used concurrently in a flock or on a site. It is essential that each bird within a flock vaccinated against ILT virus receives an effective immunising dose to ensure that there are no naive susceptible birds remaining in the flock. Serological testing can be used as an indicator of the effectiveness of vaccine administration.

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