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    • An ELISA assay for the qualitative determination of antibody levels to Canine Distemper Virus and Canine Parvovirus in canine serum or plasma samples.
    • In-clinic test with results in 20 minutes.
    • Evaluation of individual patient antibody status in the context of determining an individual vaccination program for CDV and CPV in dogs.
    • Monitoring response to vaccination following initial puppy protocol.
    • Monitoring maternal antibody levels.
    • Determination of antibody status in dogs of unknown history.
    • Determination of need to re-vaccinate dogs with history of adverse response to vaccination or where vaccination could compromise health.
    • Test performed in clinic.
    • Results in 20 minutes.
    • Tests may be run for single patients or in batches.
    • Simple colour change result.
    • Serum or plasma may be used as sample.
    • Development of blue colour in the sample wells of equal or greater intensity to the colour in the positive control well is considered a positive result and indicative of CDV SN titre ≥ 1:16 and of CPV HAI titre ≥ 1:80.
    • CDV sensitivity 94% and specificity 96% (vs SN), CPV sensitivity 91% and specificity 98% (vs HAI).1
    • 32 wells per kit (16 CDV and 16 CPV) with shelf life of 12 months.
    • Store unopened kits, unused wells and unused diluted wash solution at 2-7°C.
    • Test materials must be at room temperature (21-25°C) at time of use.
    • Samples may be stored at 2-7°C for up to 7 days prior to testing, or at -20°C or below for longer periods.
    • Severely haemolysed or lipaemic samples may produce background colour. If in doubt obtain a better quality sample.
    • Always compare sample wells to positive control. Negative control is to verify good washing technique and not for assessment of positive and negative results.
    • Washing of wells is the most important step. Wells cannot be over-washed.
    • Assessment of results should be conducted after 5 minutes of incubation, as prolonged incubation may result in non-specific colour development.
    • Hold wells against a white background for optimal assessment of colour change.
    • Do not intermix kit components from kits with different serial numbers.
    1. Studies conducted at Kansas State University, University of Wisconsin, University of Georgia and Synbiotics comprising nearly 400 samples.
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